(2) ICAAM – Instituto de Ciências Agrárias e Ambientais Mediterrâneas – Universidade de Évora
(3) Department of Animal Medicine and Surgery - Universidad CEU Cardenal Herrera
João Alves (2)
Ana Santos (1)
Patrícia Jorge (1)
Pilar Lafuente (3)
To evaluate a test protocol for the determination of the maximal lactate steady state (MLSS) in dogs, ten animals were submitted to a variable number of sessions (3-5), with a duration of 30 minutes each. Each session started with a warm-up period, after which treadmill was then set at a 5% inclination and at a constant speed, maintained for 20m. In subsequent sessions, speed was increased or decreased 0,5km/h, based on the results obtained. Blood samples were collected from the saphenous veins, and values for blood lactate (BL), heart rate (HR), rectal temperature (RT) and glycaemia were determined at rest (T0), after the warm-up period (T1) and every 5 minutes until the end of the test (T2-T5). A mean number of four sessions were required to determine the MLSS. Results of MLSS were compared with the visual lactate threshold (LTv).
Mean BL, HR, RT and glycaemia rest, after the warm-up period and at the MLSS, as significant variations, can be observed in table 1. Mean speed at MLSS was 14.6±1.47km/h and BL was 2.7±0.45mmol/L, well below 4mmol/L, the reference value for the lactate threshold. A correlation of 0.87 was observed between the MLSS and LTv.
The present results consist, to the authors’ knowledge, in the first description of a protocol to determine the MLSS in dogs. It provides valuable information for exercise monitoring and future training planning and performance evaluation. Subsequent studies are needed, in order to assess the relevance of training at the MLSS level.
BIBLIOGRAFÍA
1. Marcellin-Little DJ, Levine D, Taylor R. Rehabilitation and Conditioning of Sporting Dogs. Vet Clin North Am Small Anim Pract. 2005;35(6):1427-1439. doi:10.1016/j.cvsm.2005.08.002.
2. Svedahl K, MacIntosh BR. Anaerobic Threshold: The Concept and Methods of Measurement. Can J Appl Physiol. 2003;28(2):299-323. doi:10.1139/h03-023.
3. Garnacho-Castaño M V., Domínguez R, Ruiz-Solano P, Maté-Muñoz JL. Acute Physiological and Mechanical Responses During Resistance Exercise at the Lactate Threshold Intensity. J Strength Cond Res. 2015;29(10):2867-2873. doi:10.1519/JSC.0000000000000956.
4. Lindner AE. Maximal lactate steady state during exercise in blood of horses1. J Anim Sci. 2010;88(6):2038-2044. doi:10.2527/jas.2009-2693.
5. Goodwin ML, Harris JE, Hernández A, Gladden LB. Blood Lactate Measurements and Analysis during Exercise: A Guide for Clinicians. J Diabetes Sci Technol. 2007;1(4):558-569. doi:10.1177/193229680700100414.
6. Restan AZ, Zacche E, da Silva SB, et al. Lactate and glucose thresholds and heart rate deflection points for Beagles during intense exercise. Am J Vet Res. 2019;80(3):284-293. doi:10.2460/ajvr.80.3.284.
7. Evans D. Exercise testing in the field. In: Equine Exercise Physiology - the Science of Exercise in the Athletic Horse. Philadelphia: Saunders Elsevier; 2008:12-27.
8. Miranda M, Queiroz-Neto A, Silva-Júnior J, et al. Comparison of the lactate minimum speed and the maximal lactate steady state to determine aerobic capacity in purebred Arabian horses. N Z Vet J. 2014;62(1):15-20. doi:10.1080/00480169.2013.815103.
9. Alves JC, Santos A. Physiological, haematological and biochemical shifts in police working dogs during a riot control exercise. Comp Exerc Physiol. 2016;12(4):193-198. doi:10.3920/CEP160016.
10. Taylor S, Shmon C, Su L, et al. Evaluation of Dogs with Border Collie Collapse, Including Response to Two Standardized Strenuous Exercise Protocols. J Am Anim Hosp Assoc. 2016;52(5):281-290. doi:10.5326/JAAHA-MS-6361.
11. Frye CW, VanDeventer GM, Dinallo GK, et al. The effects of a post-exercise carbohydrate and protein supplement on repeat performance, serum chemistry, insulin and glucagon in competitive weight-pulling dogs. J Nutr Sci. 2017;6(8):e27. doi:10.1017/jns.2017.23.
