VALIDATION OF A FELINE-SPECIFIC IMMUNOASSAY FOR THE QUANTIFICATION OF SERUM AMYLOID A (SAA) IN CATS

Type:
Free Communication
Topic:
Pathology
Companies:
(1) Universidad de Murcia
Authors:
Damian Escribano Tortosa (1)
Asta Tvarijonaviciute (1)
Pablo Ruiz Herrera (1)
Luis Guillermo Gonzalez Arostegui (1)
Jose Joaquin Ceron (1)
Paper:
RESUMEN CORTO - SHORT SUMMARY
The objective of this study was to perform the validation of a new feline-specific immunoassay for in-house Serum Amyloid A (SAA) measurement (FUJI DRI-CHEM IMMUNO AU CARTRIDGE vf-SAA, FUJIFILM; Method A). An analytical validation including precision (Coefficients of Variations; CVs), accuracy (linearity under dilution and recovery) and sensitivity (limit of detection; LD) was performed. In addition, an overlap performance test was made using serum samples from cats with non-inflammatory and cats with inflammatory diseases. These tests were also performed for comparative purposes with other two previously validated methods such as the SAATIA; LZ-SAA (Eiken Chemical Co., Ltd; analyser Olympus AU600; Method B) and the SAA VET test kit (CUBE-VET analyser, Eurolyser Diagnostica GmbH; Method C) and correlations between the three methods were studied. Methods A and B were precise with high, medium and low SAA concentrations (CVs < 10% in all cases). All methods showed high linearity after dilution and recovery rates between acceptable percentages (80-120%). The assay validated was highly correlated with the other two assays tested and was able to detect the different SAA concentrations between cats with and without inflammatory conditions in the overlap performance test (p< 0.0001). Based on these results it can be stated that the feline-specific immunoassay evaluated in this study is suitable for the measurement of SAA concentration in cats.

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