CHANGES IN CANINE CREATINE PHOSPHOKINASE (CK) SERUM CONCENTRATIONS AFTER 3 DIFFERENT OVARIECTOMY TECHNIQUES
(2) INTERLAB-UMU, FACULTAD DE VETERINARIA, UNIVERSIDAD DE MURCIA
(3) BIOTECHNOLOGY OF ANIMAL AND HUMAN REPRODUCTION, INSTITUT OF FOOD AND AGRICULTURAL TECHNOLOGY, UNIVERSIDAD DE GIRONA
Belén Cuervo Serrato (1)
Javier Tórtola López (1)
Marc Yeste Oliveras (3)
José Joaquín Cerón Madrigal (2)
Mónica Rubio Zaragoza (1)
Muscle damage and inflammation are seen in many medical circumstances in dogs, and also take part of the surgical trauma in the post-operative period. Creatine phosphokinase (CK) is an enzyme found in the cytoplasm of muscle cells considered as a sensitive biomarker of muscle stress, being its elevation proportional to the amount of muscle tissue involved(1, 2).
Elective ovariectomy is frequently performed in the veterinary practice and many approaches have been described(3-5). The aim of this study is to objectively evaluate the muscle damage after three different ovariectomy techniques by means of determination of CK activity. Forty-five bitches were randomly divided into three groups: L (laparoscopic ovariectomy), M (midline ovariectomy) and F (flank ovariectomy). CK activity was measured before surgery and 1h, 24h, 72h and 168h after surgery. CK activity increased one hour after surgery in all groups although there were no significant differences between groups. At 24h post-surgery, CK levels peaked for groups M and F, being F significantly higher than M and L. At 72h levels decreased in all groups and continued decreasing until 168h post-surgery when levels reached similar values to the baseline ones. Giving consideration to CK as muscle damage biomarker, flank ovariectomy results in more muscle stress compared to midline and laparoscopic ovariectomy.
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