A 5-year-old male mixed breed dog that weighed 8,5kg was presented to consultation with chronic lameness. The patient was diagnosed with an erosive lesion of the right carpus, a mandibular fracture with teeth loss, and cranial cruciate ligament rupture of the left pelvic limb. Furthermore, additional exams, demonstrated that the patient was positive for Leishmaniasis.
Despite the poor prognosis of the osteolytic carpal lesions, a pancarpal arthrodesis with a 2,7 DCP plate was proposed and accepted by the owner. To promote bone healing, a bio-enhanced scaffold (60% hydroxyapatite + 40% -tricalcium phosphate) seeded with allogeneic mesenchymal stem cells (MSCs) enriched with autologous platelet lysate (PL) was conducted. The symphyseal mandibular fracture was treated with an interfragmentary 2.0mm screw and cruciate rupture was treated with an extracapsular technique.
Surgery and resolution of the carpal lesion were developed successfully and bone healing was completed in a short period of time (6 weeks). Additionally, the patient recovered completely from the mandibular fracture and from the cruciate injury. In this particular case, canine mesenchymal allogenic stem cells applied simultaneously to a bioenhanced bioceramic scaffold enriched with platelet lysate have demonstrated to be a safe and could be a successful treatment for enhancing bone healing in osteolytic challenging cases. More research must be performed to allow demonstration of this effectiveness in similar cases.
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