In exotic animals field, sex determination is frequently required, especially for species with poor or absent sexual dimorphism [Pellet S, Cope I, 2013]. In blue‐tongued lizards (Tiliqua scincoides) a morphometric evaluation study for sex identification was performed in adult and sub-adult specimens [Phillips CA et al., 2016], but this technique couldn’t be performed in young animals [Phillips CA et al., 2016]. The sex of two adult Tiliqua gigas has been proved using through the cloacoscopy, identifying the presence of one pair of papillae (uretheral papillae) in the urodeum in male and two pairs of papillae in the urodeum in females (two uretheral papillae, caudally and two genital papillae, cranially) [Spadola F. et al., 2021]. Currently, sex identification techniques described in BTS (blue-tongued skinks) have not relied on direct gonadal visualization. Cystoscopy in BTS allow the direct gonads visualization through the bladder wall, in transparency. Moreover morphology, development and other features could be evaluated.